7XHS

Crystal structure of CipA crystal produced by cell-free protein synthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	I 4
Unit cell lengths	61.090, 61.090, 54.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.200 - 2.110
R-factor	0.1905
Rwork	0.187
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.159
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.110	2.110
Number of reflections	5781	5781
<I/σ(I)>	9.04	1.7
Completeness [%]	100.0
Redundancy	19.3
CC(1/2)	0.994	0.587

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293	cell-free protein crystallization