7XHP

Structure of a Glucose 6-Phosphate Dehydrogenase from Zymomonas mobilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	80.610, 101.073, 321.066
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	78.180 - 2.780
R-factor	0.2394
Rwork	0.233
R-free	0.28930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e08
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.180	78.180	2.850
High resolution limit [Å]	2.780	12.430	2.780
Rmerge	0.095	0.054	0.408
Rmeas	0.117	0.067	0.532
Total number of observations	530641
Number of reflections	218096	2290	14733
<I/σ(I)>	7.43	13.49	1.56
Completeness [%]	85.4	79.7	77.9
Redundancy	2.433	2.71	1.868
CC(1/2)	0.986	0.989	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M sodium malonate (pH 6.0), 10% w/v PEG 3350