7XHJ

Crystal structure of RuvC from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2017-01-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	40.020, 72.600, 113.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.540 - 1.600
R-factor	0.2054
Rwork	0.204
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hjr
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.540	27.540	1.640
High resolution limit [Å]	1.600	7.150	1.600
Rmerge	0.050	0.027	0.355
Rmeas	0.057	0.033	0.411
Total number of observations	160024
Number of reflections	42426	148	3122
<I/σ(I)>	16.52	25.2	4.35
Completeness [%]	94.7	24.7	96.8
Redundancy	3.772	2.635	3.869
CC(1/2)	0.998	0.998	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	ammonium sulfate, BIS-TRIS, Polyethylene glycol 3350