7XHG

Crystal structure of the NTF2L domain of human G3BP1 in complex with the Caprin-1 derived peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	41.669, 171.761, 42.968
Unit cell angles	90.00, 99.82, 90.00

Refinement procedure

Resolution	85.880 - 2.460
R-factor	0.2095
Rwork	0.206
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fcj
RMSD bond length	0.012
RMSD bond angle	1.572
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.880	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.106	0.532
Rmeas	0.126	0.629
Rpim	0.068	0.333
Number of reflections	21506	1065
<I/σ(I)>	28.2	4.1
Completeness [%]	99.6
Redundancy	3.4
CC(1/2)	0.971	0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30 % w/v Polyethylene glycol 8,000