7XHG
Crystal structure of the NTF2L domain of human G3BP1 in complex with the Caprin-1 derived peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.669, 171.761, 42.968 |
| Unit cell angles | 90.00, 99.82, 90.00 |
Refinement procedure
| Resolution | 85.880 - 2.460 |
| R-factor | 0.2095 |
| Rwork | 0.206 |
| R-free | 0.27460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fcj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.572 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.880 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.106 | 0.532 |
| Rmeas | 0.126 | 0.629 |
| Rpim | 0.068 | 0.333 |
| Number of reflections | 21506 | 1065 |
| <I/σ(I)> | 28.2 | 4.1 |
| Completeness [%] | 99.6 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.971 | 0.818 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30 % w/v Polyethylene glycol 8,000 |
