7XHF

Crystal structure of the NTF2L domain of human G3BP1 in complex with the USP10 derived peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97915
Spacegroup name	P 31 2 1
Unit cell lengths	81.874, 81.874, 94.247
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.900 - 2.680
R-factor	0.2127
Rwork	0.210
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fcj
RMSD bond length	0.014
RMSD bond angle	1.728
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.900	2.730
High resolution limit [Å]	2.680	2.680
Rmerge	0.093	0.528
Rmeas	0.099	0.561
Rpim	0.034	0.187
Number of reflections	9910	528
<I/σ(I)>	57.1	5.1
Completeness [%]	92.7
Redundancy	8.8
CC(1/2)		0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1 M Sodium citrate tribasic dihydrate pH 5.0; 19 % w/v Polyethylene glycol 3,350