7XH4
Dihydrofolate Reductase-like Protein SacH in safracin biosynthesis complex with safracin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-02 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 68.727, 68.727, 157.286 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.760 - 2.200 |
| R-factor | 0.239 |
| Rwork | 0.234 |
| R-free | 0.28210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xw7 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.760 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.078 | 0.496 |
| Number of reflections | 11851 | 1186 |
| <I/σ(I)> | 55.28 | |
| Completeness [%] | 99.7 | |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 28% (w/v) PEG 1500, 100mM PCB buffer pH 7.0 |
