7XH2

Dihydrofolate Reductase-like Protein SacH in safracin biosynthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-02
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 64 2 2
Unit cell lengths	68.881, 68.881, 156.019
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.840 - 2.800
R-factor	0.2299
Rwork	0.222
R-free	0.30290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xw7
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHENIX
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.840	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.101	0.261
Number of reflections	5881	589
<I/σ(I)>	46.87
Completeness [%]	99.0
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	28% (w/v) PEG 1500, 100mM PCB buffer pH 7.0