7XH2
Dihydrofolate Reductase-like Protein SacH in safracin biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-02 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 68.881, 68.881, 156.019 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.840 - 2.800 |
| R-factor | 0.2299 |
| Rwork | 0.222 |
| R-free | 0.30290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xw7 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.840 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.101 | 0.261 |
| Number of reflections | 5881 | 589 |
| <I/σ(I)> | 46.87 | |
| Completeness [%] | 99.0 | |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 28% (w/v) PEG 1500, 100mM PCB buffer pH 7.0 |
