7XGX

Glucosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-12-25
Detector	APEX II CCD
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	75.055, 75.863, 126.644
Unit cell angles	90.00, 90.24, 90.00

Refinement procedure

Resolution	32.220 - 2.390
R-factor	0.2172
Rwork	0.215
R-free	0.26540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xgv
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.220	2.475
High resolution limit [Å]	2.390	2.390
Rmerge	0.026	0.348
Rmeas	0.036	0.492
Rpim	0.026	0.348
Number of reflections	55366	5597
<I/σ(I)>	14.72	2.39
Completeness [%]	95.3
Redundancy	2
CC(1/2)	0.999	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	PEG3350, Calcium acetate hydrate