7XGV
Legionella glucosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2020-10-30 |
| Detector | APEX II CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.181, 76.014, 126.579 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 29.220 - 2.270 |
| R-factor | 0.2073 |
| Rwork | 0.206 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jsz |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.220 | 2.351 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.022 | 0.247 |
| Rmeas | 0.032 | 0.350 |
| Rpim | 0.022 | 0.247 |
| Number of reflections | 66028 | 64406 |
| <I/σ(I)> | 16.27 | 2.6 |
| Completeness [%] | 97.3 | 92.6 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.999 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | PEG3350, Calcium acetate hydrate |
