7XGT
Crystal structure of rice OsRNase ZS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97892 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.368, 83.148, 87.147 |
| Unit cell angles | 90.00, 109.56, 90.00 |
Refinement procedure
| Resolution | 32.952 - 1.700 |
| R-factor | 0.1736 |
| Rwork | 0.172 |
| R-free | 0.20100 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14rc2_3191) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.952 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.085 | 1.380 |
| Rmeas | 0.088 | |
| Rpim | 0.022 | 0.362 |
| Number of reflections | 64632 | 6404 |
| <I/σ(I)> | 19.1 | 1.8 |
| Completeness [%] | 98.9 | 98.98 |
| Redundancy | 15.2 | 15 |
| CC(1/2) | 0.999 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 283 | 20% PEG 3350, 100 mM Na-citrate pH 5.5, 100 mM KCl |
