7XGN

Quinolinate Phosphoribosyl Transferase (QAPRTase) from Streptomyces pyridomyceticus NRRL B-2517 in complex with Nicotinic Acid (NA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2020-06-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 2
Unit cell lengths	119.208, 150.973, 55.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.460 - 2.600
R-factor	0.1944
Rwork	0.192
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qpo
RMSD bond length	0.007
RMSD bond angle	0.911
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.460	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	31172	1506
<I/σ(I)>	12.8
Completeness [%]	96.3
Redundancy	4.6
CC(1/2)	0.985	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Citric acid, pH 5.0, 1.6M Ammonium sulfate