7XFP

Crystal structure of Helicobacter pylori IceA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-29
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	41.857, 67.381, 143.175
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.510 - 1.870
R-factor	0.202
Rwork	0.200
R-free	0.23970
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.789
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.900
High resolution limit [Å]	1.870	1.870
Rmerge	0.090	0.591
Number of reflections	34219	1725
<I/σ(I)>	17.5	2.6
Completeness [%]	99.4	99.9
Redundancy	3.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	ammonium sulfate, MES