7XFF

Polysaccharide export protein Wza

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-16
Detector	AGILENT EOS CCD
Wavelength(s)	0.988
Spacegroup name	C 2 2 21
Unit cell lengths	185.768, 193.039, 106.535
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.630 - 2.300
R-factor	0.1849
Rwork	0.184
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j58
RMSD bond length	0.009
RMSD bond angle	1.217
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.630	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	76600	7
<I/σ(I)>	0.8
Completeness [%]	83.8
Redundancy	5
CC(1/2)	0.800	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	291.1	0.05 M Potassium phosphate monobasic, pH 7.3, 5% glycerol