7XFD
Polysaccharide export protein Wza
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-16 |
| Detector | AGILENT EOS CCD |
| Wavelength(s) | 0.988 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 96.895, 96.895, 220.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.520 - 2.200 |
| R-factor | 0.2666 |
| Rwork | 0.265 |
| R-free | 0.28790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j58 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.816 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.520 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 27007 | 7 |
| <I/σ(I)> | 0.8 | |
| Completeness [%] | 98.6 | |
| Redundancy | 5 | |
| CC(1/2) | 0.800 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.1 | 0.05 M Potassium phosphate monobasic, pH 7.3, 5% glycerol |
