7XF6

Crystal Structure of Human Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-02-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	33.035, 56.062, 61.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.060 - 1.300
R-factor	0.1527
Rwork	0.151
R-free	0.17100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7e02
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	28633	1358
<I/σ(I)>	40.42
Completeness [%]	99.5
Redundancy	6.4
CC(1/2)	0.989	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		277.5	Na-acetate, PEG 6K, NaCl