7XEY

EDS1-PAD4 complexed with pRib-ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	82.357, 91.863, 94.271
Unit cell angles	90.00, 111.79, 90.00

Refinement procedure

Resolution	46.070 - 2.290
R-factor	0.186
Rwork	0.184
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nfu
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.330
High resolution limit [Å]	2.290	6.210	2.290
Rmerge	0.137	0.044	0.694
Rmeas	0.149	0.048	0.783
Rpim	0.058	0.019	0.353
Total number of observations	368298
Number of reflections	57868	2981	2850
<I/σ(I)>	5.2
Completeness [%]	98.4	98.3	98
Redundancy	6.4	6.6	4.5
CC(1/2)		0.999	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	0.04 M citric acid, 0.06 M Bis-Tris propane pH 6.4, 20% w/v polyethylene glycol 3350