7XE5

T4 lysozyme mutant-S44C/C54T/N68C/A93C/C97A/T115C, pH4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	60.295, 60.295, 96.872
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.290 - 1.300
R-factor	0.1536
Rwork	0.153
R-free	0.17120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vnr
RMSD bond length	0.006
RMSD bond angle	0.977
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.300	1.350
High resolution limit [Å]	1.300	1.300
Rmeas	0.081	0.591
Number of reflections	50560	4785
<I/σ(I)>	26	2.6
Completeness [%]	99.6	95.9
Redundancy	10.1	6.6
CC(1/2)	0.998	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	A crystal was soaked into precipitant-free solution (pH4)