7XDS
Crystal structure of wheat stem rust effector AvrSr35
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.122, 115.273, 143.162 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.890 - 2.060 |
| R-factor | 0.2584 |
| Rwork | 0.257 |
| R-free | 0.28690 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.702 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.890 | 2.134 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.142 | 0.142 |
| Number of reflections | 55016 | 55016 |
| <I/σ(I)> | 19 | |
| Completeness [%] | 97.8 | |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1 M sodium malonate (pH 6.0), 12% (v/v) PEG 3350 |
