7XDR

Crystal structure of a glucosylglycerol phosphorylase from Marinobacter adhaerens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	102.267, 185.174, 257.619
Unit cell angles	90.00, 101.56, 90.00

Refinement procedure

Resolution	33.400 - 2.400
R-factor	0.1961
Rwork	0.194
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s9v
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.400	33.400	2.550
High resolution limit [Å]	2.400	7.140	2.400
Rmerge	0.118	0.038	0.789
Rmeas	0.128	0.041	0.854
Total number of observations	4861714
Number of reflections	363096	27301	114265
<I/σ(I)>	12.73	39.63	2.12
Completeness [%]	99.6	99.3	98.2
Redundancy	6.776	6.497	6.793
CC(1/2)	0.997	0.999	0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	PEG8000, MgAc