7XCD
Crystal Structure of the Disease-Specific Protein of Rice Stripe Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 45.327, 81.311, 122.037 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.591 - 1.710 |
| R-factor | 0.1815 |
| Rwork | 0.180 |
| R-free | 0.19580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7xcc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.827 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 10.000 | 1.740 |
| High resolution limit [Å] | 1.710 | 4.640 | 1.710 |
| Rmerge | 0.115 | 0.071 | 1.261 |
| Rmeas | 0.125 | 0.077 | 0.990 |
| Rpim | 0.047 | 0.029 | 0.642 |
| Total number of observations | 148523 | ||
| Number of reflections | 23810 | 1210 | 927 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 95.6 | 89.4 | 74.9 |
| Redundancy | 6.2 | 6.5 | 3.8 |
| CC(1/2) | 0.996 | 0.279 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | PEG 8000, KCl, Tris |
