7XCD

Crystal Structure of the Disease-Specific Protein of Rice Stripe Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	45.327, 81.311, 122.037
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.591 - 1.710
R-factor	0.1815
Rwork	0.180
R-free	0.19580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xcc
RMSD bond length	0.007
RMSD bond angle	0.827
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	10.000	1.740
High resolution limit [Å]	1.710	4.640	1.710
Rmerge	0.115	0.071	1.261
Rmeas	0.125	0.077	0.990
Rpim	0.047	0.029	0.642
Total number of observations	148523
Number of reflections	23810	1210	927
<I/σ(I)>	8.4
Completeness [%]	95.6	89.4	74.9
Redundancy	6.2	6.5	3.8
CC(1/2)		0.996	0.279

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	PEG 8000, KCl, Tris