7XCC

Crystal Structure of the Disease-Specific Protein of Rice Stripe Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	C 2 2 21
Unit cell lengths	59.408, 74.193, 78.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.374 - 1.750
R-factor	0.1697
Rwork	0.165
R-free	0.20800
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.370
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.740
High resolution limit [Å]	1.710	4.640	1.710
Rmerge	0.119	0.091	0.537
Rmeas	0.128	0.098	0.607
Rpim	0.046	0.037	0.271
Total number of observations	146645
Number of reflections	18988	1030	798
<I/σ(I)>	13.5
Completeness [%]	99.2	98.3	86
Redundancy	7.7	7.1	4.4
CC(1/2)		0.992	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	PEG 3350, Ammonium acetate, HEPES Sodium