7XCA
Crystal structure of the porcine astrovirus capsid spike domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.480, 105.170, 47.470 |
Unit cell angles | 90.00, 111.91, 90.00 |
Refinement procedure
Resolution | 44.040 - 2.500 |
R-factor | 0.1836 |
Rwork | 0.178 |
R-free | 0.23410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.782 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.040 | 2.589 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 14608 | 2840 |
<I/σ(I)> | 15.81 | |
Completeness [%] | 99.6 | |
Redundancy | 2 | |
CC(1/2) | 0.998 | 0.958 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.5 | 293 | 0.2M magnesium chloride, 25% PEG 3350, 0.1M Bis-Tris |