7XA1

Thermotoga maritima ferritin variant-Tm-E(S111Y)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.276911
Spacegroup name	P 32 1 2
Unit cell lengths	56.609, 56.609, 128.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.640 - 2.200
R-factor	0.2312
Rwork	0.226
R-free	0.27940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.640	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	12639	649
<I/σ(I)>	7.44
Completeness [%]	91.5
Redundancy	1.1
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25mM HEPES pH 7.5, 35% 1,4-dioxane