7XA0

Thermotoga maritima ferritin variant-Tm-E(S111F)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 32 1 2
Unit cell lengths	55.337, 55.337, 128.818
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.940 - 2.030
R-factor	0.2076
Rwork	0.203
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	autoPROC
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.940	2.103
High resolution limit [Å]	2.030	2.030
Number of reflections	15978	1498
<I/σ(I)>	21.51
Completeness [%]	98.7
Redundancy	1.1
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM MES pH 6.0, 200mM Lithium sulfate, 35% MPD