7X9S

Crystal structure of a complex between the antirepressor GmaR and the transcriptional repressor MogR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	91.870, 91.992, 268.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.490 - 3.110
R-factor	0.1974
Rwork	0.195
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fdq 7x9r
RMSD bond length	0.007
RMSD bond angle	0.888
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.160
High resolution limit [Å]	3.110	3.110
Number of reflections	43043	2094
<I/σ(I)>	16.7	1.3
Completeness [%]	99.9	100
Redundancy	4.5
CC(1/2)	0.993	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 4000, ammonium acetate, glycerol, sodium citrate