7X9L
Crystal structure of MutT-8-oxo-dGTP complex: Reaction for 4 hr using 10 mM Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-01 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.531, 56.106, 59.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.320 - 1.820 |
| R-factor | 0.1538 |
| Rwork | 0.152 |
| R-free | 0.18110 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3a6t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.165 |
| Data reduction software | HKL-2000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.320 | 1.890 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.071 | 0.247 |
| Number of reflections | 11698 | 1078 |
| <I/σ(I)> | 19.8 | |
| Completeness [%] | 97.7 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | potassium sodium tartrate, sodium citrate, ammonium sulfate |
