7X9I

Crystal structure of MutT-8-oxo-dGTP complex: Reaction for 12 hr using 5 mM Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-25
Detector	RIGAKU JUPITER 210
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	38.239, 56.004, 59.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.140 - 1.900
R-factor	0.1467
Rwork	0.144
R-free	0.18960
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3a6t
RMSD bond length	0.006
RMSD bond angle	1.342
Data reduction software	HKL-2000
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.140	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.056	0.160
Number of reflections	10412	980
<I/σ(I)>	32.5
Completeness [%]	99.3
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	potassium sodium tartrate, sodium citrate, ammonium sulfate