7X98
5-Aminolevulinate synthase HemA from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-12-02 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.157, 68.896, 85.639 |
| Unit cell angles | 82.02, 85.26, 66.06 |
Refinement procedure
| Resolution | 34.610 - 2.050 |
| R-factor | 0.1544 |
| Rwork | 0.152 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bwn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.569 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 4.420 | 2.050 |
| Rmerge | 0.065 | 0.048 | 0.178 |
| Total number of observations | 303616 | ||
| Number of reflections | 82589 | 8325 | 8084 |
| <I/σ(I)> | 14.2 | ||
| Completeness [%] | 94.3 | 95 | 92.6 |
| Redundancy | 3.7 | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | Bis-Tris pH 6.5, Polyethylene glycol monomethyl ether 2000 |
