7X8V
Cooperative regulation of PBI1 and MAPKs controls WRKY45 transcription factor in rice immunity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-09 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.932, 89.780, 107.088 |
| Unit cell angles | 90.00, 106.56, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.840 |
| R-factor | 0.19466 |
| Rwork | 0.193 |
| R-free | 0.22363 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.342 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.418 | |
| Number of reflections | 141131 | 6824 |
| <I/σ(I)> | 34.3 | |
| Completeness [%] | 98.0 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.918 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 8% (w/v) PEG 8000, 0.1M HEPES, pH 7.0, 0.2M sodium citrate |
