7X8U
Crystal structure of the N-terminal Solanaceae domain of the Sw-5b NLR immune receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2020-05-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 81.530, 81.530, 98.445 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.400 - 2.890 |
| R-factor | 0.2507 |
| Rwork | 0.249 |
| R-free | 0.26630 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.789 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.490 | 2.910 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.124 | 1.471 |
| Number of reflections | 9900 | 1438 |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 18.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium bromide, 0.2 M potassium thiocyanate, 0.1 M Tris-HCl pH 7.8, 2.6% gamma-PGA (Na+ form, LM), 2.4% w/v PEG20000 |
