7X8T
Frizzled 10 CRD in complex with hB9L9.3 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.456, 100.794, 99.149 |
| Unit cell angles | 110.07, 89.94, 113.79 |
Refinement procedure
| Resolution | 46.060 - 2.510 |
| R-factor | 0.1777 |
| Rwork | 0.176 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cm4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.293 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.108 | 0.058 | 0.345 |
| Rmeas | 0.128 | 0.068 | 0.415 |
| Rpim | 0.068 | 0.037 | 0.227 |
| Total number of observations | 290369 | ||
| Number of reflections | 83801 | 4207 | 4163 |
| <I/σ(I)> | 5.3 | ||
| Completeness [%] | 98.7 | 99.1 | 98 |
| Redundancy | 3.5 | 3.4 | 3.3 |
| CC(1/2) | 0.991 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1 M Na citrate, pH 5.0, 15% PEG 8000 |
