7X7L
Crystal structure of ZmHPPD-Y13161 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.952, 83.428, 85.610 |
| Unit cell angles | 89.78, 97.46, 107.64 |
Refinement procedure
| Resolution | 45.615 - 1.887 |
| R-factor | 0.1747 |
| Rwork | 0.173 |
| R-free | 0.20750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sp8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.976 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.887 | 1.887 |
| Rmerge | 0.105 | 0.316 |
| Number of reflections | 109886 | 8132 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 92.7 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.976 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Sodium acetate 4.6, 8% w/v PEG 4000 |
