7X7K
Ancestral L-Lys oxidase (AncLLysO-2) L-Arg binding form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.258, 77.214, 93.258 |
| Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
| Resolution | 45.200 - 1.600 |
| Rwork | 0.152 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7x7i |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.739 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 140292 | 18995 |
| <I/σ(I)> | 26.6 | |
| Completeness [%] | 98.1 | |
| Redundancy | 6.6 | |
| CC(1/2) | 1.000 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350 |
