7X7J

Ancestral L-Lys oxidase (AncLLysO-2) L-Lys binding form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	79.408, 77.400, 93.414
Unit cell angles	90.00, 104.36, 90.00

Refinement procedure

Resolution	45.300 - 1.400
Rwork	0.158
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7x7i
RMSD bond length	0.014
RMSD bond angle	1.867
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.300	1.470
High resolution limit [Å]	1.400	1.400
Number of reflections	215621	29666
<I/σ(I)>	27.2
Completeness [%]	99.1
Redundancy	6.4
CC(1/2)	1.000	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350