7X7H

Crystal structure of Fructose regulator/Histidine phosphocarrier protein complex from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-16
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	33.940, 62.355, 312.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.130 - 2.000
R-factor	0.1809
Rwork	0.179
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rzr
RMSD bond length	0.009
RMSD bond angle	0.939
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.996	0.061	0.801
Rmeas	0.138		0.855
Rpim	0.036		0.224
Number of reflections	46109	4327	4221
<I/σ(I)>	6.6		4.733
Completeness [%]	100.0	99.6	100
Redundancy	7.6	7.3	7.6
CC(1/2)	0.998		0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	calcium acetate, PEG 3350, spermidine