7X7D

SARS-CoV-2 Delta RBD and Nb22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-09
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	151.824, 108.557, 115.310
Unit cell angles	90.00, 126.23, 90.00

Refinement procedure

Resolution	61.230 - 2.920
R-factor	0.2079
Rwork	0.205
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cjf
RMSD bond length	0.009
RMSD bond angle	1.100
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20-4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	30832	3106
<I/σ(I)>	6.9
Completeness [%]	93.8
Redundancy	3.2
CC(1/2)	0.985	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	0.1 M Tris (pH 7.0), 37.5% Jeffamine