7X78
L-fuculose 1-phosphate aldolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 92.614, 92.614, 41.828 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.780 - 1.850 |
| R-factor | 0.1694 |
| Rwork | 0.164 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fua |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.791 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rpim | 0.027 | 0.142 |
| Number of reflections | 15994 | 787 |
| <I/σ(I)> | 25.66 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.5 | 13.1 |
| CC(1/2) | 0.992 | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.01 M MgCl2, 0.05 M Tris pH 7.5, 1.6 M (NH4)2SO4. |
