7X70

TRIM7 in complex with C-terminal peptide of NSP8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 65
Unit cell lengths	80.100, 80.100, 53.127
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.220 - 1.250
R-factor	0.1433
Rwork	0.142
R-free	0.17030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7w0q
RMSD bond length	0.009
RMSD bond angle	1.120
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.220	1.295
High resolution limit [Å]	1.250	1.250
Number of reflections	46056	2099
<I/σ(I)>	15.96
Completeness [%]	85.9
Redundancy	2
CC(1/2)	0.999	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	Potassium sodium tartrate, Lithium sulfate, Tris-HCl