7X6Z

TRIM7 in complex with C-terminal peptide of NSP12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 65
Unit cell lengths	80.038, 80.038, 53.285
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.200 - 1.430
R-factor	0.1448
Rwork	0.143
R-free	0.17220
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.792
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.200	1.480
High resolution limit [Å]	1.430	1.430
Number of reflections	35358	6895
<I/σ(I)>	15.56
Completeness [%]	98.2
Redundancy	2
CC(1/2)	0.999	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	DL-Malic acid, PEG3350