7X6Y

TRIM7 in complex with C-terminal peptide of NSP5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 65
Unit cell lengths	79.915, 79.915, 53.083
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.160 - 1.390
R-factor	0.1438
Rwork	0.143
R-free	0.16540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7w0q
RMSD bond length	0.005
RMSD bond angle	0.871
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.160	1.440
High resolution limit [Å]	1.390	1.390
Number of reflections	39139	3870
<I/σ(I)>	11.44
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.997	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	Potassium sodium tartrate tetrahydrate, Tris