7X5P

Truncated VhChiP (1-19aa) in complex with doxycycline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	110
Detector technology	CCD
Collection date	2020-08-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.92
Spacegroup name	P 1
Unit cell lengths	92.393, 92.500, 96.522
Unit cell angles	101.11, 101.14, 109.72

Refinement procedure

Resolution	20.020 - 3.400
R-factor	0.1862
Rwork	0.182
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mdo
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.020	3.400
High resolution limit [Å]	3.400	3.400
Rmeas	0.086
Rpim	0.061
Number of reflections	36533	36533
<I/σ(I)>	53.9
Completeness [%]	95.0
Redundancy	1.9
CC(1/2)	0.942	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.075 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 8, and 22 % w/v PEG 1500