7X5F

Nrf2-MafG heterodimer bound with CsMBE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-03
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	245.171, 54.686, 85.952
Unit cell angles	90.00, 99.18, 90.00

Refinement procedure

Resolution	45.270 - 2.600
R-factor	0.2276
Rwork	0.226
R-free	0.25920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a5t
RMSD bond length	0.005
RMSD bond angle	0.754
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.270	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.043	0.753
Number of reflections	34821	3266
<I/σ(I)>	29.18
Completeness [%]	99.2	93.78
Redundancy	7.3	6.1
CC(1/2)	1.000	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	50 mM MES monohydrate (pH 5.6), 20 mM KCl, 10 mM MgSO4 heptahydrate, 7%(v/v) PEG400