7X5B
Crystal structure of RuvB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-07-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 65 |
| Unit cell lengths | 85.677, 85.677, 76.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.040 - 2.160 |
| R-factor | 0.1775 |
| Rwork | 0.173 |
| R-free | 0.21730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6blb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.908 |
| Data reduction software | HKL-3000 (1.13_2998) |
| Data scaling software | HKL-3000 (1.13_2998) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.140 | |
| Number of reflections | 17198 | |
| <I/σ(I)> | 23.9 | |
| Completeness [%] | 99.6 | |
| Redundancy | 20 | |
| CC(1/2) | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M LProline, 0.1 M HEPES pH 7.5, 10% w/v polyethylene glycol 3350, 10 mM Trimethylamine hydrochloride |
