7X4P
CD-NTase EfCdnE in complex with intermediate pppUpU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-11-02 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.332, 56.777, 64.780 |
| Unit cell angles | 90.00, 97.24, 90.00 |
Refinement procedure
| Resolution | 27.960 - 1.600 |
| R-factor | 0.1507 |
| Rwork | 0.149 |
| R-free | 0.18840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7x4c |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.035 | 0.068 |
| Rpim | 0.021 | 0.040 |
| Number of reflections | 38096 | 3716 |
| <I/σ(I)> | 33.1 | 18.9 |
| Completeness [%] | 96.6 | 95 |
| Redundancy | 3.9 | 3.9 |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | sodium tartrate, PEG 3350, MgCl2, UTP |
