7X3N

Crystal structure of anti-mPEG h15-2b Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-06-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	63.930, 112.572, 158.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.610 - 2.240
Rwork	0.205
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l7i
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.240	2.240
Number of reflections	55882	6846
<I/σ(I)>	12.7
Completeness [%]	99.7	99.6
Redundancy	7.1
CC(1/2)	0.926	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	18% (w/v) PEG 6000, 1% (w/v) PEG 2000 MME, 0.15 M lithium sulfate monohydrate, 0.1 M citric acid, pH 3.5