7X3H
The structure of Clostridium botulinum ParM in the apo conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-21 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.635, 51.103, 64.917 |
Unit cell angles | 90.00, 115.28, 90.00 |
Refinement procedure
Resolution | 32.000 - 1.700 |
Rwork | 0.192 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.716 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.030 | 0.473 |
Rpim | 0.018 | 0.343 |
Number of reflections | 35734 | 2896 |
<I/σ(I)> | 37.7 | 1.8 |
Completeness [%] | 97.5 | |
Redundancy | 3.6 | |
CC(1/2) | 0.736 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 5 mg/ml CBg-ParM mutant 1 mM AMPPNP 0.2 M ammonium chloride 22% (w/v) PEG 3350 |