7X2B
Red fluorescent protein from Diadumene lineata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.929, 75.394, 118.955 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.001 - 1.630 |
| Rwork | 0.171 |
| R-free | 0.20540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vk1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.674 |
| Data reduction software | XDS |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.650 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.068 | 0.788 |
| Number of reflections | 26779 | 1298 |
| <I/σ(I)> | 13 | 2.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 3.0 M Ammonium Sulfate, 0.1 M Immidazole, 2.5 mg/mL protein |
