7X15

Crystal structure of MIGA2 LD targeting domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9794
Spacegroup name	P 61 2 2
Unit cell lengths	100.126, 100.126, 163.941
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.880 - 2.852
R-factor	0.2017
Rwork	0.200
R-free	0.23900
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.744
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Number of reflections	21478	1055
<I/σ(I)>	18
Completeness [%]	100.0
Redundancy	21.1
CC(1/2)	0.997	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	18% SOCKALAN cp5, 100 mM HEPES pH7.0, 300 mM ammonum formate