7X14

Crystal structure of phospho-FFAT motif of MIGA2 bound to VAPB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-15
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	33.363, 44.507, 83.761
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.695 - 1.675
R-factor	0.1885
Rwork	0.186
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ikk
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.695	1.730
High resolution limit [Å]	1.675	1.675
Number of reflections	14734	715
<I/σ(I)>	25.9
Completeness [%]	99.8
Redundancy	6.3
CC(1/2)	0.995	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	1.8 M ammonium sulfate, 100 mM phosphae-citrate pH 4.2, 2% Iso-propanol