7X0P
Crystal structure of sugar binding protein CbpD from Clostridium thermocellum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 69.910, 63.170, 69.700 |
Unit cell angles | 90.00, 106.20, 90.00 |
Refinement procedure
Resolution | 33.467 - 1.500 |
R-factor | 0.1711 |
Rwork | 0.170 |
R-free | 0.19990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xss |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.467 | 33.467 | 1.540 |
High resolution limit [Å] | 1.500 | 6.710 | 1.500 |
Rmerge | 0.043 | 0.023 | 0.513 |
Rmeas | 0.059 | 0.033 | 0.685 |
Total number of observations | 167390 | ||
Number of reflections | 86939 | 651 | 6443 |
<I/σ(I)> | 10.3 | 23 | 1.91 |
Completeness [%] | 94.7 | 62.7 | 95.2 |
Redundancy | 1.925 | 1.747 | 1.937 |
CC(1/2) | 0.997 | 0.996 | 0.685 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 0.1 M Sodium acetate, pH 4.5, 20% w/v Polyethylene glycol 3350 |